By L. S. Dzung, W. Rohrbach (auth.), L. S. Dzung, W. Rohrbach (eds.)
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Additional resources for Enthalpie-Entropie-Diagramme für Wasserdampf und Wasser
Biotechniques 8:58–60 42. Mesulam MM (1978) Tetramethyl benzidine for horseradish peroxidase neurohistochemistry: a non-carcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents. J Histochem Cytochem 26:106–117 43. Evans NA, Hoyne PA (1982) A fluorochrome from aniline blue: structure, synthesis and fluorescence properties. Aust J Chem 35: 2571–2575 44. Pal MK (1965) Effects of differently hydrophobic solvents on the aggregation of cationic 40 Aleš Soukup dyes as measured by quenching of fluorescence and/or metachromasia of the dyes.
Observe in bright field optics. Polyanionic compounds stain intensely red. Limited penetration of the dye was reported, which should be considered evaluating the results on thicker sections. 6 Aniline Blue Fluorochrome (Sirofluor) Staining 1. Stain the sections for 5–10 min in solution of aniline blue or flood sections with solution of Sirofluor (stock diluted 1:50 in distilled water or suitable buffer). Aqueous solutions low in ionic solutes decrease background staining of cellulose . 2. Rinse carefully with water or suitable buffer.
5. Mount into 75 % glycerol (sections could be also dehydrated and mount permanently). 6. Observe in bright field optics. Polyanionic compounds stain cyan color. 5 Ruthenium Red Staining 1. Wash fresh or fully rehydrated sections with water or suitable buffer. 2. Control sections might be methylated to block free carboxyl groups in acidified methanol (1 M HCl in MetOH) for 4 h at 60 °C . Methylation should mask free carboxyl and therefore inhibit polyanionic staining. Methylation of the carboxyl can be reverted with alkalized ethanol (1 % KOH in 70 % EtOH, 10 min in laboratory temperature).