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Extra info for Embedded Research Tick1 Tiny CMOS Keyer
The lagging strand is synthesized by a difficult to dissociate complex containing DNA polymerase alpha and primase (Fig. 13a,b; Downey et al. 1988; De Pamphilis et al. 1988). The identity, exact number of subunits and the properties of this complex depend on the organism studied, method of extraction, and tissue used for the preparation. We are far from understanding the details of its structure and function (Fry and Loeb 1986). In the case of replicational synthesis of the lagging DNA strand of the SV 40 virus, the picture which emerges is the following (Fig.
An asymmetry in the composition of DNA strands. In the case of circular DNA molecules this asymmetry would be expected to start at the origin of replication, to be uniform up to the middle of the molecule, and to switch polarity at the point where the leading and the lagging strands meet each other. 2. Depending on the distribution of the priming "hot spots" (Hay et al. 1984) one expects to find compositional fluctuations and the fluctuations of the rate of accumulation of mutations related to the patterns of alternating leader and trailer sub segments of Okazaki pieces.
15. The distribution of assymetries is the base composition of the two strands of several mammalian DNA sequences. The areas of the bars are proportional to the occurrence of whichever base is in excess of its complement. The diagrams are for mouse adult E miTIm globin, mouse immunoglobin y2b heavy chain constant region, simian virus 40 and mouse polyoma virus strain A2. The polyoma virus numbering system is in the reverse orientation but the data are presented in the figure so that they are comparable.