By Wilfred D. Stein
For college students in addition to researchers this e-book describes the fascinating new advances within the molecular biology of delivery proteins and integrates this knowledge with delivery kinetics, functionality, and legislation. Experimental information are associated with thought.
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Additional info for Channels, Carriers, and Pumps: An Introduction to Membrane Transport
T h e thin lines r e p r e s e n t the levels of c o n c e n t r a t i o n of t h e solute S. hm I I m 44 2. Simple Diffusion of Nonelectrolytes and Ions partition coefficient. ) T h e c o n c e n t r a t i o n gradient that drives the diffusion of the solute within the m e m b r a n e is the concentration difference 5 i , - S n , w h e r e this e x p r e s s i o n refers to the concentrations in the m e m b r a n e adja cent to the bathing solution. At either face of the m e m b r a n e , the p e r m e a n t S will be in equilibrium across the interface b e t w e e n the m e m b r a n e interior and the adjacent bath ing solution.
T h e t u b e c a n b e c u t w i t h a s h a r p b l a d e so that t h e cells c a n b e selectively r e m o v e d a n d their c o n t e n t of t h e p e r m e a n t m e a s u r e d . F r o m t h e rise (or loss) in this c o n t e n t with time a n d a k n o w l e d g e of t h e total surface a r e a of t h e cells, o n e c a n calculate the permeability coeffi cient. 48 2. Simple Diffusion of Nonelectrolytes and Ions across the cell m e m b r a n e virtually ceases [Fig. 6(c)]. This m e t h o d can fail if S is too soluble in the organic p h a s e .
5) and c o m p a r e the resulting ion distributions for the same Ai//. T h u s with a 59-mV transbarrier poten tial, side I positive to side II, a cation will h a v e to be at a 10-fold higher concentration at side II t h a n at side I to be in equilibrium, while an anion will h a v e to b e at a 10-fold lower concentration at side II than at side I for it also to be in equilibrium. 2)], both ions often being not far from equilibrium with the electrical potential across the m e m b r a n e . If the c o n c e n t r a t i o n gradient for S is not that given by E q .