Biosynthesis, 1st Edition by E. P. Abraham, G. G. F. Newton (auth.), David Gottlieb, Paul

By E. P. Abraham, G. G. F. Newton (auth.), David Gottlieb, Paul D. Shaw (eds.)

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SPRECHER, D. B. SPRINSON, and B. D. DAVIS: The biosynthesis of shikimic from D-glucose. J. BioI. Chern. 220,477 (1956). STRATTON, CH. , and M. C. REBSTOCK: A new metabolite of Streptomyces venezuelae D-threo-1-p-aminophenyl-2-dichloroacetamido-1 ,3-propanediol. Arch. Biochem. Biophys. 103, 159 (1963). , T. TAZAKI, H. OKAMI, and S. FUKUYAMA: Isolation of a crystalline antibiotic substance from a strain of streptomyces and its identity with chloromycetin. Japan. Med. J. 1, 358 (1948). VINING, L. , and D.

4%. The direct incorporation of phenylalanine-P4C into gliotoxin was firmly established by degradation of the gliotoxin to indole-2-carboxylic acid (IV). Decarboxylation of the indole-2-carboxylic acid to indole and CO 2 demonstrated that all of the carbon-14 resided in the carboxyl group. :I ~NACOOH ~ Indole + COz H (IV) Further proof that all nine carbon atoms of phenylalanine, and not merely the aliphatic side chain, were incorporated into gliotoxin was provided by the isolation of gliotoxin-3 H after phenylalanine-U-3 H was added to growing cultures of T.

296, 18 (1963). , L. C. VINING, and R H. HASKINGS: Biogenetic studies on volucrisporin. Can. J. Chern. 40, 2357 (1962). , and R G. CHENOWETH: Biosynthesis of gliotoxin. 1. Incorporation of phenylalanine-1- and _2_14C. J. Am. Chern. Soc. 80, 4391 (1958). WEINDLING, R: Trichoderma lignorum as a parasite of other soil fungi. Phytopathology 22, 837 (1932). WEINDLING, R, and O. H. EMERSON: The isolation of a toxic substance from the culture filtrate of Trichoderma. Phytopathology 26, 1068 (1936). WINSTEAD, J.

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