Assessment of Immune Status by the Leukocyte Adherence by D. M. P. Thomson

By D. M. P. Thomson

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Extra resources for Assessment of Immune Status by the Leukocyte Adherence Inhibition Test

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The overall view of the sequence of the LAI reaction which Creemers finally concluded to best explain her observations was as follows: (1) antigen reacts with members of the adherent population (probably macrophages) and a lymphokine is released (SF^; (2) SFX reacts with T cells which respond by releasing SF 2 ; (3) SF 2 , according to this scheme, stimulates adherent cells which produce LAIF; and (4) the LAIF acts on neighboring leukocytes finally to inhibit their capacity for adherence. The sequence is shown schematically below: Adherent cell SFi SF2 adherent cell LAIF target leukocytes lose adhesiveness Thus, a total of three soluble factors seem required in this system.

1978) but in agreement with Koppi et al. (1979). Additional evidence for an obligate T cell requirement was adduced from the observation that polyvinylpyrolidone, a T cell-independent antigen, gave excellent antibody production but could not predispose spleen cells to give an LAI 38 I. LAI on Hemocytometers reaction. This was later confirmed by Powell et al. (1978) working with a similar type of antigen (DNP-Ficoll) in the hemocytometer assay of rat cells. , 1979), serum from tumor-bearing mice "armed" normal spleen cells making them LAI reactive.

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