Advances in Blood Substitutes: Industrial Opportunities and by Robert M. Winslow M.D. (auth.), Robert M. Winslow M.D., Kim

By Robert M. Winslow M.D. (auth.), Robert M. Winslow M.D., Kim D. Vandegriff Ph.D., Marcos Intaglietta Ph.D. (eds.)

Each bankruptcy of this quantity is a contribution from a professional within the box, selected by means of the editors to give a contribution to the 1997 "Current matters in Blood alternative examine and improvement" path given in San Diego, March 17-19. The members have been chosen due to their services in parts which the editors think to be severe to the development of the sector, and which replicate task in "hot" components of proper examine. whereas there's a continuity common for the once a year direction, every year brings alterations in emphasis and content material. In earlier years, we have been usually unable to supply time for individuals to provide their perspectives and reviews. as a result, this yr we inspired dialogue after every one presentation. those classes have been recorded, transcribed, and are revealed with the chapters herein. We think that the product is especially with reference to the shooting this year's direction in print, and belief readers will take pleasure in analyzing the consistently candid and infrequently provocative comments from the viewers. the fee paid for inclusion of the dialogue transcriptions was once a hold up in ebook. every one writer was once allowed to edit his/her dialogue part in addition to the ultimate model of the chapters ahead of e-book. The adjustments are frequently for grammar, and we attempted, while attainable, to not modify the conversational type of those interchanges.

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So perhaps you could tell me if that is a first-time demonstration, and what mediators might be regulating this rapid change? I know you've published on the role of nitric oxide via the intravascular side of things. Do you think this is a nitric oxide mediated phenomenon? Baldwin: Well, this is the first time. I haven't seen anyone else publish this kind of work. As far as nitric oxide, that's a good point. We did do some studies where we used nitric oxide inhibitors, and we looked at the same thing, and we did find a change in the fenestrae when we inhibited nitric oxide, they did seem to disappear.

50 Jain Based on perfusion rates, four regions can be recognized in a tumor: an avascular, necrotic region, a semi-necrotic region, a stabilized microcirculation region, and an advancing front (Endrich et al. 1979). Intra-tumor blood flow distributions in spontaneous animal and human tumors are now being investigated using nuclear magnetic resonance, positron emission tomography (Melder et al. 1993), and functional computed tomography (Eskey et al. 1992, Eskey et al. 1994, Hamberg et al. 1994).

We haven't done that yet. We've done that with the studies on FITC albumin. Hirsch: And, the last question. I've labeled hemoglobin with fluorescent probes and often you see changes in oxygen affinity which would indicate conformational changes also. Did you measure the oxygen affinity change of the FITC-labeled hemoglobin? Baldwin: No, we didn't. Phillips: Have you looked at the effect of PEG by itself or else PEG linked to other protein molecules, and have you seen these effects with any other kind of proteins?

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